Media Gallery

Fluorescence Microscope Simulation of the Mitotic Spindle

February 6th, 2009  |  Published in Media Gallery

The mitotic spindle model (Yeh et al., 2008). (a) Side-on view; (b) end-on view. Helices represent the chromatin labeled with green fluorescing protein. Experimental and noise-free simulated images in side-on orientation (c-d) and end-on orientation (e-f). Differences in background between experimental and simulated images are caused by external chromatin not accounted for in the model. Structural variation in real specimens account for shape discrepancies.

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Mitotic Spindle

January 22nd, 2009  |  Published in Media Gallery

Model of the mitotic spindle and an isosurface from a 3D stack of fluorescence images of a real mitotic spindle. The model has been manually placed relative to the image data. A "make it better" button in the FluoroSim module of the CISMM Microscope Simulator will optimize the fit of the geometric model to the image information by forward simulation of the fluorescence microscope imaging process.

Model of the mitotic spindle and an isosurface from a 3D stack of fluorescence images of a real mitotic spindle. The model has been manually placed relative to the image data.

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Fibrin Networks

January 22nd, 2009  |  Published in Media Gallery

Here is an example of a series of fibrin networks formed between ridges of optical glue.  Perhaps they formed from a sheet?

Here is an example of a series of fibrin networks formed between ridges of optical glue. Perhaps they formed from a sheet?

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Fibrin Sheets

January 22nd, 2009  |  Published in Media Gallery

Molecularly thin fibrin sheets have been observed during experiments in our group.  There is some evidence that these sheets develop into fibrin networks.  Here is one image showing perhaps this exact phenomenon.  A sheet is laying across ridges made of hardened optical glue.  However the sheet appears to be separating into individual fibers.

Molecularly thin fibrin sheets have been observed during experiments in our group. There is some evidence that these sheets develop into fibrin networks. Here is one image showing perhaps this exact phenomenon. A sheet is laying across ridges made of hardened optical glue. However the sheet appears to be separating into individual fibers.

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What a Protein Looks Like

January 22nd, 2009  |  Published in Media Gallery

movie to come: what_protein_looks_like4.mov

caption:

This visualization, produced in 1982 and narrated by Duke Biochemist Jane Richardson, shows many ways to visualization the protein Copper-Zinc Superoxide Dismutase. Jane explains the benefits of providing many different visualization for the same system. Each visualization has its own strengths and weaknesses, making it suitable for some tasks and not others. Taken together, they provide a more complete understanding of the protein. (475 Megabytes, may take awhile to load)

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Adenovirus with DNA

January 22nd, 2009  |  Published in Media Gallery

This image of adenovirus on a silicon substrate was scanned using the nanoManipulator. An adenovirus capsid has disrupted, spilling its DNA onto the surface in a tangle. The portion of the image above a certain height has been made semitransparent by adding an opacity texture. This enables us to see through the image to the icosahedral model of an adenovirus drawn with the virus in the upper left corner. Surface color is according to height. The surface has a slight slope, resulting in uniform blue, gray, and red areas.

This image of adenovirus on a silicon substrate was scanned using the nanoManipulator. An adenovirus capsid has disrupted, spilling its DNA onto the surface in a tangle. The portion of the image above a certain height has been made semitransparent by adding an opacity texture. This enables us to see through the image to the icosahedral model of an adenovirus drawn aligned with the virus in the upper left corner. Surface color is according to height. The surface has a slight slope, resulting in uniform blue, gray, and red areas.

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A Tracked Strand of DNA

January 21st, 2009  |  Published in Media Gallery

A strand of DNA tracked using cores. The red curve is the core. The blue curves are the DNA edges implied by the core location and radius.

A strand of DNA tracked using cores. The red curve is the core. The blue curves are the DNA edges implied by the core location and radius.

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3DFM User Interface

January 21st, 2009  |  Published in Media Gallery

This shows the 3 Dimensional Force Microscope's User Interface design as of 9/25/2002. It shows the tracked bead in green, the surface carved out by the bead as it traveled, a yellow trace of the bead's path, and a video image of the sample including the bead. We are continually adding to the system to meet the needs of particular experiments.

This shows the 3 Dimensional Force Microscope

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Isosurface of Flourescence

January 21st, 2009  |  Published in Media Gallery

This image shows a 3D isosurface of fluoresence in a fixed human ephethelial lung cell culture. The lower surface is of the cells and cilia; the upper is the mucus layer above.

This image shows a 3D isosurface of fluoresence in a fixed human ephethelial lung cell culture. The lower surface is of the cells and cilia; the upper is the mucus layer above.

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Multivariate Visualization

January 21st, 2009  |  Published in Media Gallery

Three data sets are shown in this AFM image: topography is mapped directly to height, friction is mapped to color, and areas of high adhesion (normal to surface) are circled by contour lines.

Three data sets are shown in this AFM image: topography is mapped directly to height, friction is mapped to color, and areas of high adhesion (normal to surface) are circled by contour lines.

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