Segmented vesicle witih proteins

This image (and linked movie) shows a rotating 3D view of a vesicle that was semi-automatically segmented from a 3D TEM image reconstructed from a tilt series.  A handful of seed points were placed in one slice of the image and the 3D vesicle was automatically extracted.  The image also shows a very preliminary automatic segmentation of proteins extending through the vesicle wall; the extent of these proteins is currently clipped by an arbitrary global parameter setting.

Simulated image generation in FluoroSim is fast enough to enable interaction with specimen models while watching real-time updates of the expected fluorescence image. This video shows some of FluoroSim’s main capabilities.

Cilia-driven mucus flow visualization by David Borland

David Borland developed a flow visualization technique and used the output of Brian Eastwood’s ImageTracker program to construct this visualization of cilia-driven mucus flow on a human lung cell culture video from David Hill.  He overlaid the flow on the cell background determined by ImageTracker.

Flow speed is encoded both by color (blue lowest, through gray to red).  Flow direction is along the lines and in the direction pointed to by the arrows.

Slice through epithelial cell culture.

The image to the right shows one frame from a stack of images taken of a human lung epithelial cell culture.  The well-separated spots were thought to be cross sections through cilia sticking out the tops of cells.  There are some bright spots on the labeled cells and others (near the left of the image) that were thought to be above cells.

ImageSurfer 3D view of epithelial cells

The ImageSurfer image shown below to the right revealed that they were in fact isolated blobs of dye that were outside of the cells.