CISMM

Download imagesurfer dendritic_spines.zip

This data is from Alain Burette at Richard Weinberg’s laboratory at the University of North Carolina at Chapel Hill. The DiO data shows the stained membrane of a dentrite, with spines coming out of it. The PMCA2 data set shows where calcium channels are.

Unzip the file to a directory of your choosing. To load a data set, click File -> Open, and navigate to the directory containing the unzipped data. There are groups of images: DiO and PMCA2. To load the DiO data, double click on dio...tif. Double click on TIFF Volume File Format in the pop-up dialog. Click the Apply button in the Properties panel on the left. A white wire frame should show up, representing the bounding box of the DiO data.

To load the PMCA data set, click File -> Open again, double click on pmca2...tif, double click on TIFF Volume File Format, and finally, hit Apply. Pmca is now also showing in the Pipeline Browser.