Slice through epithelial cell culture.

The image to the right shows one frame from a stack of images taken of a human lung epithelial cell culture.  The well-separated spots were thought to be cross sections through cilia sticking out the tops of cells.  There are some bright spots on the labeled cells and others (near the left of the image) that were thought to be above cells.

ImageSurfer 3D view of epithelial cells

The ImageSurfer image shown below to the right revealed that they were in fact isolated blobs of dye that were outside of the cells.

The mitotic spindle model (Yeh et al., 2008). (a) Side-on view; (b) end-on view. Helices represent the chromatin labeled with green fluorescing protein. Experimental and noise-free simulated images in side-on orientation (c-d) and end-on orientation (e-f). Differences in background between experimental and simulated images are caused by external chromatin not accounted for in the model. Structural variation in real specimens account for shape discrepancies.

Model of the mitotic spindle and an isosurface from a 3D stack of fluorescence images of a real mitotic spindle. The model has been manually placed relative to the image data.